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猪链球菌2型的分离与鉴定

Isolation and Identification of Swine Streptococus suis Serotype 2

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【作者】 高巍韩文瑜雷连成高尚庆杜崇涛

【Author】 GAO Wei,HAN Wen-yu,LEI Lian-cheng,et al(College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China)

【机构】 吉林大学畜牧兽医学院预防兽医系吉林大学畜牧兽医学院预防兽医系 (长春130062)(长春130062)

【摘要】 目的对从四川省某猪场分离到的5株细菌进行系统鉴定。方法观察菌体的形态、培养特性、动物致病性和生化反应,并采用PCR法鉴定其血清型和毒力因子。结果5株细菌均为猪链球菌2型,均有毒力因子MRP和EF,其中S1株、S2株和S5株对BALB/c小鼠有致病性,另2株无致病性。结论该猪场的疫情是由猪链球菌2型引起的。本研究为进一步预防和控制猪链球菌2型的流行提供了依据。

【Abstract】 Objective To identify the 5 strains of swine Streptococus suis isolated from a pig farm in Sichuan Province,China.Methods Observe the morphology,culture characteristics,pathogenicity in animals and biochemical reactions of the 5 strains of swine Streptococus suis,and identify their serotypes and virulence factors by PCR.Results All the 5 strains were identified as swine Streptococus suis serotype 2 and contained virulence factors MRP and EF,of which strains S1,S2 and S5 showed pathogenicity in BALB/c mice while the other 2 strains showed no pathogenicity.Conclusion The pestilence in the pig farm was caused by swine Streptococus suis serotype 2,which provided a basis for further prevention and control of infection with swine Streptococus suis serotype 2.

【关键词】 猪链球菌2型分离鉴定
【Key words】 Swine Streptococus suis serotype 2IsolationIdentification
  • 【文献出处】 中国生物制品学杂志 ,Chinese Journal of Biologicals , 编辑部邮箱 ,2007年06期
  • 【分类号】S852.611
  • 【被引频次】3
  • 【下载频次】236
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