【摘要】 目的研究乙型脑炎病毒减毒株SA14-14-2在小鼠体内的动态分布。方法将SA14-14-2株分别经脑内和皮下途径接种小鼠,采用BHK21细胞病毒蚀斑法检测接种后不同时间病毒在小鼠不同脏器中的分布。同时以不同稀释度的强毒株SA14为对照。结果SA14-14-2株经脑内感染小鼠后,除在脑组织中短时间检出病毒外,在其他组织中均未检出病毒;经皮下感染小鼠后,在任何组织中均未检出病毒。而强毒株SA14无论经脑内还是皮下途径感染小鼠,在各种脏器中均可检出病毒。结论SA14-14-2株经皮下感染小鼠后,病毒不能在体内复制,证明乙型脑炎病毒减毒活疫苗具有较好的安全性。更多还原

【Abstract】 Objective To study the dynamic distribution of attenuated Japanese encephalitis virus(JEV) strain SA14-14-2 in mice.Methods Inject mice with JEV strain SA14-14-2 by intracerebral and subcutaneous routes respectively, using virulent JEV strain SA14 as control. Determine the JEV in various organs including brain, liver, spleen, lung, heart, muscle and kidney of mice by plaque formation test with BHK21 cells on days 1-7, 14, 21 and 28 respectively.Results After injection with SA14-14-2 strain by intracerebral route, JEV was found only in brains of mice on days 1-6, but not found in any other organs. However, after injection with strain SA14-14-2 by subcutaneous route, no JEV was detected in any organs. As a contrast, high JEV titers were detected in various organs of mice injected with SA14 strain, either by intracerebral or by subcutaneous route.Conclusion JEV SA14-14-2 strain could not be replicated in the tissues of mice after subcutaneous infection, which proved the safety of live attenuated JE vaccine.更多还原