【摘要】 目的克隆新的小鼠激活素受体相互作用蛋白5(ActRIP5)基因。方法应用酵母双杂交技术,筛选出与激活素ⅡA型受体(ActRⅡA)相互作用蛋白的基因片段,再以此基因片段作为探针,从小鼠脑cDNA文库中钓取ActRIP5cDNA。采用哺乳动物细胞双杂交系统,进一步确定ActRIP5与ActRⅡA的相互作用。采用RT-PCR检测ActRIP5mRNA在组织中的转录。结果克隆的ActRIP5全编码基因长1839bp,编码145个氨基酸残基,与ActRⅡA具有特异结合作用。ActRIP5mR-NA在小鼠多种组织中均可检出。结论ActRIP5属于ActRIP家族新成员,可以与ActRⅡA相互作用。更多还原

【Abstract】 Objective To clone a novel gene encoding mouse activin receptor-interacting protein 5.Methods Screen the gene encoding mouse activin receptor ⅡA(ActRⅡA)-interacting protein by double hybridization in yeast cells.Extract ActRIP5 cDNA from murine brain cDNA library using the screened gene fragment as a probe.The interaction between ActRIP5 and ActRⅡA was further confirmed by double hybridization in mammalian cells.The transcription of ActRIP5 mRNA in various tissues were tested by RT-PCR.Results The cloned ActRIP5 gene fragment,at a length of 1 839 bp,encoded 145 amino acid residues.The double hybridization in mammalian cells proved specific binding of ActRIP5 to ActRⅡA.ActRIP5 mRNA was detected in various murine tissues.Conclusion As a new member of ActRIP family,ActRIP5 showed interaction with ActRⅡA.更多还原