【摘要】 以绿色木霉(Trichoderma viride)、康氏木霉(Trichoderma koningii)和二株未知木霉菌株(Trichoderma spp.)为供试木霉菌株,采用对峙培养法测定了不同温度处理下对棉花黄萎病菌(Verticilliam dahliae)的拮抗作用。结果表明,木霉在不同温度下对棉花黄萎病菌的抑制作用不一,其中以30℃和25℃黑暗条件下木霉对棉花黄萎病菌菌丝生长的抑制作用最强,对峙培养5d后,绿色木霉、康氏木霉和二株未知木霉菌在30℃条件下对棉花黄萎病菌的抑制率分别达到67.3%、65.9%、56.8%、65.9%,25℃条件下的抑制率分别为65.9%、72.9%、65.9%、78.8%;20℃下木霉菌株对棉花黄萎病菌的抑制作用次之,10℃、15℃和35℃条件下木霉菌丝扩散速度较慢,且孢子产生量少,不能有效地抑制棉花黄萎病菌菌丝的扩展,表明环境温度过高或过低对木霉菌丝的生长及分生孢子的产生均有较大影响。该研究为筛选棉花黄萎病菌更为有效的生防木霉菌株提供理论依据。更多还原

【Abstract】 The antagonism experiment by face-to-face-culturing on PDA media indicated that the mycelium inhibitory efficiencies of Trichoderma sp. against Verticillium dahliae were much related to the culturing temperature. Mycelium inhibitory efficiencies varied with the temperature of 10℃,15℃,20℃,25℃, 30℃ and 35℃ in the dark. At 30℃, inhibitory efficiencies of Trechoderma sp. against Verticillium dahliae reached 67.3%, 65.9%,56.8%,65.9%, after 5 days and 65.9%,72.9%65.9% 78.8% at 25℃, respectively. The mycelium of Trichoderma. expanded very slow and no obvious control effects against Verticillium dahliae at 10℃,15℃ and 35℃. The experiment evidence was provided for looking for effective Trichoderma sp. against Verticillium dahliae.更多还原