【摘要】 运用正交设计方法,利用植物组织、细胞培养技术,成功地建立了驱蚊草组织培养快繁技术体系。研究出:不定芽诱导的最适培养基为MS+6-BA0.2mg/L+NAA0.3mg/L,增殖倍数为6.73;生根培养基为1/2MS+NAA0.4mg/L,平均每株生根数为11.2条,生根率为90.0%。通过对其离体茎段的培养研究实验,得出驱蚊香草的最适愈伤组织诱导培养基为MS+2,4-D0.5mg/L+6-BA2.0mg/L+NAA0.3mg/L,发愈率为93.3%,愈伤组织多为淡黄色,质地疏松。更多还原

【Abstract】 Based on taking the orthogonal design experiments and by using the technique of plant tissue culture, the experiment-system of tissue culture and rapid propagation of Mozzie buster was successfully established. The result showed that: the most suitable medium for the initiation of adventitious buds was MS medium supplemented with 0.2 mg/L 6-BA and 0.3 mg/L NAA, the ratio of buds propagation was 6.73; The best medium for the rooting of shoots was 1/2 MS medium supplemented with 0.4 mg/L NAA , the average number of roots per shoot was 11.2 and the rate of rooted shoots was 90.0%. The culture of the detached stems of Mozzie buster plantlets was studied, Result showed that the optimal medium for the inducement of callus tissues was MS medium supplemented with 0.5 mg/L 2 , 4-D, 2.0mg/L 6-BA and 0.3mg/L NAA , the rate of callus tissue inducement was 93.3% and the callus tissues were mostly yellowish and loose in texture.更多还原