【摘要】 作为神经突触中介导囊泡融合的核心蛋白,SNARE(soluble NSF attachment protein receptors)复合体由VAMP、syntaxin和SNAP25三种蛋白组成。而由其可溶性部分组成的SNARE核心复合体是发挥融合功能的关键部分。通过表达SNARE蛋白,应用分子筛和离子交换柱等纯化方法,重组得到了高纯度的SNARE核心复合体,并分析了其在水溶液中的稳定性。最后,采用负染电镜观察方法得到了该复合体的二维平均结构。在水溶液中,SNARE复合体核心部分为2.5 nm宽、12 nm长的棒状结构。更多还原

【Abstract】 As the key player which mediated the vesicle fusion in neuronal synapsome,SNARE(soluble NSF attachment protein receptors) complex consists of syntaxin,SNAP25,and VAMP.The SNARE core complex,which forms by the soluble part of SNARE proteins,plays the key part in fusion process.Using many purification methods,including ion exchange column,size exclusion column,SNARE core complex of high purity was got and its stability was analyzed in water solution.At last,the structure of SNARE core complex was determined using two-dimensional average method by electronic microscopy.SNARE core complex is a rod-like structure,about 2.5 nm in width and 12 nm in length.更多还原