【摘要】 RNA干扰(RNAinterference,RNAi)是指由21～23个核苷酸组成的双链RNA(dsRNA)所引发的生物细胞内同源基因转录后沉默的现象,是生物体在进化过程中普遍存在的一种基因调控机制。目前对由乙型肝炎病毒(HBV)引起的病毒性肝炎尚无令人满意的治疗效果,而RNA干扰技术的出现为各类慢性HBV感染的治疗开辟了新的途径。本文对RNA干扰抑制HBV复制及基因表达的研究现状、存在问题及应用前景进行了综述。更多还原

【Abstract】 RNA interference(RNAi) is a newly discovered phenomenon of post-transcriptional gene silence which is provoked by dsRNA. The dsRNA is initially cleaved by Dicer into 21-23 nt small interfering RNA(siRNA) and can then specifically target homologous mRNA for degradation by cellular ribonucleases. RNA interference is a common mechanism of gene regulation in biosystem evolutionary process. Current therapies for chronic hepatitis caused by hepatitis B virus (HBV) are limited. RNA interference may constitute a new therapeutic strategy for various hepatitis virus infections. In this review the advances in inhibition of HBV by RNAi, the unresolved questions and the therapeutic potential of RNAi were presented.更多还原