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土壤和空气中纤维素酶高产菌株的筛选

Isolation of High Cellulase-Producing Culture Strain in Soil and Air

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【作者】 解玉红宋文华葛艳辉曹杨贾记红强艳艳冯炘

【Author】 XIE Yu-hong, SONG Wen-hua, GE Yan-hui, CAO Yang, JIA Ji-hong, QIANG Yan-yan, FENG Xin The Environmental and Safety School, Tianjin Technology Universty, Tianjin 300191, China

【机构】 天津理工大学环境科学与安全工程学院天津理工大学环境科学与安全工程学院 天津300191天津300191

【摘要】 目的:分别从土壤和空气中分离纤维素分解菌株,以提高纤维素利用率。方法:将分离得到的菌株进行纯培养,并用刚果红染色,找出能出现透明圈的菌株,测定透明圈直径与菌落直径比,筛选产纤维素酶的菌株。结果:通过对比,从分离到的6株菌株中选出产纤维素酶活力高的2株,并对其进行鉴定,初步确定一株为曲霉属真菌,一株为放线菌。结论:本研究为提高纤维素利用率提供了微生物资源,为相关后续研究提供了物质和实验基础。

【Abstract】 Objective: In order to result the problem of environmental pollution and crisis of energy resourse, the utilization and transformation of carbohydrate may contribute a lot to the human being. Methods: The microbe strains were isolated from the soil and air. The strain of separation will be a separate training. Using Congo red staining method, the strains producing a transparent circle were identified, and the transparent circle diameter/colony diameter was calculated. Results: 6 strains were obtained, in which 2 high cellulase-producing strains were identified, one strain belonged to fungi(Asperigillus), and the other belonged to actinomycete. Conclusion: The above results give some basis for the study of utilization and transformation of carbohydrate.

【关键词】 纤维素纤维素酶菌株筛选鉴定
【Key words】 carbohydratecellulaseculture isolationidentification
【基金】 天津市科委自然科学基金项目(043611111)
  • 【文献出处】 生物技术通讯 ,Letters in Biotechnology , 编辑部邮箱 ,2007年01期
  • 【分类号】Q93-33
  • 【被引频次】8
  • 【下载频次】763
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