【摘要】 目的:丹参酮Ⅱ-A是丹参的一种重要成分。研究丹参酮Ⅱ-A的抗炎症机制。方法:以小鼠腹腔巨噬细胞系RAW264.7作为药物刺激靶细胞,使用不同浓度的分析纯丹参酮Ⅱ-A对RAW264.7细胞系进行刺激,在细胞被刺激24、48h后,使用MTT比色法和半定量RT-PCR法对刺激后的细胞进行检测。结果:通过MTT比色法检测,发现丹参酮Ⅱ-A抑制炎症细胞的增殖,初步计算出丹参酮Ⅱ-A的半抑制浓度(IC50)为43.2μmol/L;在半定量RT-PCR实验中发现,在发生炎症后,丹参酮Ⅱ-A通过抑制磷脂酶A2来减轻炎症。结论:在炎症发生后,丹参酮Ⅱ-A通过抑制磷脂酶A2来达到其抗炎症的作用。更多还原

【Abstract】 Objective: To research the anti-inflammatory mechanism of tanshinoneⅡ-A, which is an important ingredient in Salvia miltiorrhiza Bunge. Methods: RAW264.7 cell lines were inhibited by tanshinoneⅡ-A with different concentration as target cell. When RAW264.7 were inhibited for 24, 48 h, used MTT assay and semi-quantitative RT-PCR. Results: According to MTT assay, IC50 of the anti-inflammatory is 43.2 μmol/L; in semi-quantitative RT-PCR experiment, tanshinoneⅡ-A inhibit the action of phospholipases A2. Conclusion: The results also demonstrate the action of anti-inflammatory mechanism is performed by inhibiting the action of phospholipases A2.更多还原