【摘要】 目的:从真菌中分离纯化单峰物质,并对其抗人巨细胞病毒活性进行初步研究。方法:发酵获得菌丝体,通过丙酮/乙酸乙酯提取、硅胶柱层析、高效液相色谱制备,从菌丝体中获得单峰物质,经紫外光谱、质谱分析,采用细胞病变效应(CPE)抑制法,通过中性红染色计算病毒滴度,跟踪其抗病毒活性。结果:分离到的单峰物质(命名为jn219)紫外吸收峰为220nm,相对分子质量为519,半数中毒浓度(TD50)为50μg/mL,半数抑毒浓度(IC50)为1μg/mL,抑毒指数(TI)为50。结论:初步证实单峰物质jn219在体外具有抗人巨细胞病毒的活性。更多还原

【Abstract】 Objective: To separate a category of matter from a kind of fungi and discuss its activity of anti-human cytomegalovirus. Methods: The hyphostroma was obtained in fermentation and extracted by acetone/ethyl acetate, SepaxHP-Silica. The purified matter was identificted in high-performance liquid chromatograph and electrospray ionization. Its antivirus activity was detected in cytopathic effect inhabition staining of neutral red. Results: The purified matter(named jn219) was identified, and its absorption peak was 220 nm, its molecular weight was 519 Dalton, 50% toxic dose was 50 μg/mL, 50% effcetive concentration was 1 μg/mL, therapeutic index was 50. Conclusion: Jn219 is confirmed initially that it has the anti-human cytomegalovirus activity.更多还原