【摘要】 以小麦叶肉细胞原生质体-激发子互作为研究体系,通过免疫荧光标记和Ca²⁺荧光染料的装载并结合药理学试验,借助激光共聚焦扫描显微镜观察,探讨小麦抵抗叶锈菌侵染过程中微管骨架和Ca²⁺之间的内在联系。试验结果表明,激发子处理可引起抗性品种原生质体[Ca²⁺]_cyt的升高并诱发微管骨架的解聚,预解聚微管骨架,再用激发子处理,可使抗性品种原生质体[Ca²⁺]_cyt的升高幅度增加。更多还原

【Abstract】 By using immunofluorescence labeling technique and incubating the Fluo-3 AM of Ca²⁺ fluorescein,wheat（Triticum aestivum） cultivars Lovrin 10 and 5389 were selected as mesophyll pro- toplast material interaction with elicitor in this paper for studying the relationship between the pattern of microtubule and [Ca²⁺]_cyt.Confocal Laser Scanning Microscopy（CLSM） was used and pharmacologic examination was set.The results indicated:when treated with elicitor,the cytosolic calcium in the protoplasts from the Lovrin 10 was increasing and the pattern of microtubule showed depolymerization. In the protoplasts which MTs were depolymerized by oryzalin prior to elicitor treatment,the increase of cytosolic calcium was even higher than that in the one which was not depolymerized.更多还原