【摘要】 <正>环境刺激的信号转导是植物信号转导的一个重要研究方向。热激反应(heat-shockresponse,HSR)是动植物细胞或器官在遇到外界热刺激时所产生的一种保护性反应,是正常的蛋白质合成受阻时产生热激蛋白(heat-shockprotein,HSP)的一种细胞生理活动,其表达通过热休克转录因子(heat-shock factor,HSF)来进行调控[1]。编码热激蛋白基因的启动子区域存在着一段保守的DNA序列,是热休克转录因子的结合位点(heat-shockelement,HSE)。当植物受到外界的热刺激时,HSF可以与HSE特异性结合,激活热激蛋白基因的表达,更多还原

【Abstract】 As a crucial transcription factor family,heat-shock factors were mainly analyzed and characterized in tomato and Arabidposis. In this study,we isolated a putative heat-shock factor OsHSF13 that interacted specifically with heat-shock element(HSE) from Oryza sativa L. by yeast one-hybrid method. The full-length cDNA of OsHSF13 has a 1047bp open reading frame(ORF). It has HSF special DNA-binding domain(DBD) and oligomerization domain(OD). Analysis of the deduced amino acid sequences revealed that OsHSF13 was a class C heat shock factor(HSF) with all the conserved sequence elements characteristic of heat stress transcription factor. Bioinformatic analysis showed that the sequence and structure of OsHSF13′s DNA binding domain had a high similarity with LpHSF24.更多还原