【摘要】 在植物应对伤害等环境刺激的反应中,已知茉莉酸(JA)作为一种重要的信号分子在植物体内长距离运输,但目前对JA的细胞和亚细胞定位知之甚少。本研究用免疫荧光显微镜技术和免疫胶体金电镜技术证明茉莉酸分布在蚕豆叶片叶肉细胞的叶绿体、表皮细胞的细胞壁、保卫细胞的细胞壁、细胞质、叶绿体和细胞核上。其中保卫细胞的叶绿体和细胞核是JA分布的主要场所。叶片的局部灼伤可提高JA在质外体和气孔保卫细胞中的水平。由此推测,伤胁迫下JA分配的改变可能与植物体防御反应密切相关,并参与了对气孔运动的调控。更多还原

【Abstract】 Higher plants have to cope with environmental stimulus such as wounding. Jasmonic acid (JA) is an essential long-distance signaling compound. There is rare information about JA cellular and subcellular localization by now. In this work, using the immuno-fluorescence and immuno-gold electron microscopy, distributions of JA were determined in different cells of Vicia faba leaf. It showed that JA existed in the epidermal cells, mesophyll cells and guard cells, mainly localized in the cytosol and chloroplast of mesophyll cells, cell wall of epidermal cells, and cytosol, cell wall, chloroplast and nucleus of guard cells. Wounding increased JA accumulation in the apoplast and guard cells. Our results suggest that JA plays an important role as a signal in the defense response and involves in regulation of the stomatal movement in response to wounding stress.更多还原