【摘要】 为了探讨航天与常规诱变技术对MT工程菌株的影响,进行了HNO2.UV、γ-射线、LiCl及γ-射线+LiCl分别与航天共诱变对MT工程菌株活性的影响试验,并且就航天对MT工程菌株的致死作用及质粒的稳定性进行了研究。结果表明:MT工程菌株经航天处理后,活菌数降低,死亡率为34.59%,航天对MT工程菌株具有一定的致死作用;MT工程菌株经UV、γ-射线、LiCl及γ-射线+LiCl分别与航天共诱变处理后的生长活性没有改变,MT工程菌株经HNO2与航天共诱变处理后的生长速率减慢;在航天处理的作用下,MT工程菌株的质粒不易丢失,能够维持很好的稳定性,丢失率仅为8.26%,而置于地面的MT工程菌株的质粒易丢失,丢失率为83.51%。更多还原

【Abstract】 In order to probe into the effect of co-mutation by space and ordinary technology on MT engineering strain,a study on the effect of space mutation with HNO2,UV,γ-irradiation,LiCl and γ-irradiation together with LiCl was conducted.The results showed that space mutation would lead 34.59% MT engineering strain to death.The activities didn’t change when MT engineering strain were mutated by space with UV,γ-irradiation,LiCl and γ-irradiation together with LiCl,but the activities changed when MT engineering strain space was mutated by space with HNO2.The plasmid in engineering strain could be kept stead by space.更多还原