【摘要】 目的:提高甘薯的遗传转化率,为建立快速高效的甘薯遗传转化体系奠定基础。方法:以甘薯多个优良栽培品种无菌苗茎切段为受体,利用农杆菌LBA4404/pSP10Z做介导,研究了影响甘薯转化的几个因素。结果:接种侵染时间对转化效率影响很大,以2~15min为宜,最高转化率可达32.14%;不同基因型的转化受体之间转化率差别较大;在培养过程中最后30min加入终浓度为50μmol/L AS的接种菌侵染的外植体,转化率较对照提高了2.02倍;共培养培养基中加入终浓度为50μmol/L的AS,转化率较对照组提高了6.11倍;AS终浓度为50μmol/L同时pH为4.8的共培养培养基有利于转化的发生,转化率较对照组提高了1.73倍;共培养培养基中脯氨酸的添加并不能提高转化率。结论:该研究为快速高效甘薯遗传转化体系的建立提供了依据。更多还原

【Abstract】 Objective: Sweetpotato transformation efficiency would been raised.Methods: In this paper,the stem explants of many superior cultivars of sweetpotato and LBA4404/pSP10Z strain of Agrobacterium tumefaciens were used as test material.The effect of many factors on sweetpotato genetic transformation had been studied.Results: Inoculation duration had effect on efficiency of transformation.The suitable time for inoculation duration was 2～15min.The highest frequency of transformation was 32.14%.The transformed receptors of different genotypes had different sensitivity to the same Agrobacterium strain.Transformation efficiency had been raised 2.02 times when 50μmol/L acetosyringone was presence in culture of bacterium at the last 30min.Transformation efficiency had been raised 6.11 times when 50μmol/L acetosyringone was presence in co-culture medium.The pH 4.8 of co-culture medium could raise transformation efficiency too.The transformation efficiency had been raised 1.73 times.Conclusion: This paper supplied some gist for the establishment of fast and efficient genetic transformation system of sweetpotato mediated by A.tumefacienens.更多还原