【摘要】 目的:探寻无患子愈伤组织诱导的最佳外植体、培养基和培养条件。方法:采用5因素混合水平正交设计,用DPS2000统计软件对其结果进行方差分析和多重比较(SSR)。结果:诱导愈伤组织的最佳外植体为当年生枝条水培芽的嫩茎段,用0.2%HgCl2消毒2min,其无菌存活率为98.33%,培养条件为光照或黑暗,最佳培养基配方为:MS(B5)+6-BA 1.5mg.l-1+NAA0.2mg.l-1,其愈伤组织的诱导率为97.78%。结论:激素配比是影响愈伤组织诱导的主要因素。较高的6-BA浓度(1.5~2.5mg.l-1)与较低的NAA浓度(0.2mg.l-1)配比,有利于无患子愈伤组织的诱导。更多还原

【Abstract】 Objective:It was to search after the optimal explants,medium and condition of callus inducement of Sopindus mukorossi Gaertn.Methods: The Orthogonal design L8(4×24) was used in this experiment and the statistic software DPS2000 was employed to analyse the result’s variance and multiple comparisons.Results: The results showed that the tender stem on the twig which grew in sterile water was best explants,which was sterilized with 0.2% HgCl2 for 2min.Its surviving rate was 98.33%;Inducing condition was darkness or light;the optimal medium was MS(B5)+6-BA 1.5mg·l-1+NAA 0.2mg·l-1,On which callus induction rate was 97.78%.Conclusion: The influence of the combination of 6-BA and NAA on callus induction was a key.A considerable 6-BA concentration(1.5～2.5mg·l-1)in combination with a little NAA(0.2mg·l-1)would avail callus induction.更多还原