【摘要】 目的:采用6份卡瓦胡椒材料、21份栽培胡椒和野生胡椒材料1、份不同属的草胡椒材料共计28份试验材料,开发1对特异SCAR引物。方法:在对它们进行了RAPD研究的基础上,通过克隆、测序和引物设计进行了SCAR分子标记研究。结果:研究开发了1对卡瓦胡椒特异SCAR引物P4.1和P4.2,用这对特异引物对试验的28份材料进行PCR扩增,结果只有6份卡瓦胡椒材料扩增出了预期大小562bp的特异带,其它材料均无任何扩增。结论:这说明引物P4.1和P4.2为卡瓦胡椒特异SCAR引物,可用于卡瓦胡椒的分子鉴定,这对卡瓦胡椒种质资源的真伪鉴定有一定帮助。更多还原

【Abstract】 Objective:This research used 6 materials of kava,21 materials of cultivated pepper and wild pepper,1 material of Peperomia pellucida kunth.,added up to 28 test materials,in order to develop a SCAR molecular marker.Methods: On the basis of RAPD analysis,we conducted the study of SCAR molecular marker by cloning,sequencing and designing primers.Results: This test developed a pair specific primers of P4.1 and P4.2.Using the specific primers P4.1 and P4.2 in the PCR,only 6 kava materials amplified the 562bp specific band,the others were not any amplified.Conclusion: This test showed that P4.1 and P4.2 could apply to molecular identify of kava germplasm materials.P4.1 and P4.2 have some use in the true and false molecular identify of kava.更多还原