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聚氨酯泡沫半固定化培养盘基网柄菌
Immobilized cultivation of Dictyostelium discoideum with polyurethane foam
【摘要】 研究了聚氨酯泡沫应用于固定化盘基网柄菌的可行性,发现以简单处理过的聚氨酯泡沫为载体,能够高效实现盘基网柄菌的固定化培养。考察了载体粒径大小、载体量和摇床转速等对固定化培养的影响,在优化的培养条件和固定化条件下,盘基网柄菌的最大细胞密度是悬浮培养的2~4倍。
【Abstract】 The immobilization of D.discoideum with polyurethane foam(PUF) was proposed and the effects of PUF’s properties on the immobilization was examined.The results showed that PUF was a suitable carrier for the efficient imoblization of D.discoideum.The effects of PUF addition and culture conditions were optimized to attain high density of D.discoideum.Under optimized culture conditions,high cell density of D.discoideum(4.56×107 cells/mL) has been achieved in the shake flasks,which was 2～4 times higher than that of free-cell culture in the axenic medium.
【基金】 国家自然科学基金资助项目(30370039)
- 【文献出处】 生物加工过程 ,Chinese Journal of Bioprocess Engineering , 编辑部邮箱 ,2007年02期
- 【分类号】Q93-335
- 【被引频次】7
- 【下载频次】161