【摘要】 从临床收集耐亚胺培南的铜绿假单胞菌和鲍曼不动杆菌共50株,进行头孢他啶和2-巯基乙醇的表型协同试验(CAZ+ME),然后进行金属酶IMP-1基因的PCR检测。选取IMP-1阳性株测序,用PCR方法检测有无一类整合子基因(IntI1)。表型的检测发现有28株为协同阳性,其中铜绿假单胞菌27株,鲍曼不动杆菌1株。PCR和测序检测出其中一株铜绿假单胞菌含有IMP-1基因,同时也含有IntI1基因。首次在中国西部地区发现产IMP-1型金属酶、同时也含有一类整合子的铜绿假单胞菌,对于临床上研究细菌的耐药性传播具有重要意义。更多还原

【Abstract】 A total of 50 clinical imipenem-resisitant isolates of Pseudomonas aeruginosa and Acinetobacter baumannii were subjected to the ceftazidime-2-mercaptoethanol -double-disk synergy test and to the PCR assays with primers specific for blaIMP-1.After the process of sequencing the positive one to identify the results, PCR analysis was conducted with primers specific for class 1 integrons. For synergy test, 28 isolates gave positive results, among which were 27 Pseudomonas aeruginosa and Acinetobacter baumannii. Only one Pseudomonas aeruginosa was found to carry blaIMP-1, and blaInt1 at the same time. This is the first ascertainment of IMP-1 producing Pseudomonas aeruginosa isolate carrying blaIntI1 in West China,which is of significantce to the research on the clinical spread of these drug-resisitant genes.更多还原