【摘要】 探讨不同月龄、去卵巢对大鼠骨髓间充质干细胞(MSCs)向脂肪细胞分化能力的影响。选用健康3月龄和6月龄Spraque-Dawley(SD)雌性大鼠行双侧卵巢切除术,建立骨质疏松症的动物模型;使用密度梯度离心法分别获取各组骨髓间充质干细胞(MSCs),体外培养传至3代,加入成脂肪诱导液进行诱导,油红O染色观察细胞内脂滴;并用RT-PCR法检测成脂肪分化标志物脂蛋白脂酶(LPL)mRNA表达水平。实验分为8组:1)3月龄正常大鼠骨髓间充质干细胞组(MSCs3control);2)3月龄去卵巢骨质疏松大鼠骨髓间充质干细胞组(MSCs3ovx);3)6月龄正常大鼠骨髓间充质干细胞组(MSCs6control);4)6月龄去卵巢骨质疏松大鼠骨髓间充质干细胞组(MSCs6ovx);5)3月龄正常大鼠骨髓间充质干细胞成脂肪诱导组(MSCs ADI3control);6)3月龄去卵巢骨质疏松大鼠骨髓间充质干细胞成脂肪诱导组(MSCs ADI3ovx);7)6月龄正常大鼠骨髓间充质干细胞成脂肪诱导组(MSCs ADI6control);8)6月龄去卵巢骨质疏松大鼠骨髓间充质干细胞成脂肪诱导组(MSCs ADI6ovx)。结果显示:(1)年老组大鼠MSCs成脂肪细胞分化能力大于年幼组大鼠;(2)成脂肪诱导后,成脂肪诱导各组MSCs胞浆内均有脂滴形成,MSCs ADI3ovx和MSCs ADI6ovx明显多于相应对照组。(3)成脂诱导后,与相应的对照组相比,MSCs ADI3ovx和MSCs ADI6ovx LPL mRNA表达水平明显增高。研究结果表明,年老组和去卵巢骨质疏松大鼠(MSCs)向脂肪细胞分化能力皆增强。更多还原

【Abstract】 In order to study the effect of ovariectomy on the capacity of rat MSCs’ differentiation into adipocytes,we established the animal models of osteoporosis by performing ovariectomy on the 3-months-old and 6-month-old female SD rats.Then MSCs were isolated from these rats by means of the density-gradient centrifugation method.After adipogenic induction(ADI),lipid droplets were detected by oil red O staining,and the mRNA level of lipoprotein lipase(LPL)was measured by RT-PCR assay.The experiments included 8 groups:1)MSCs from 3-month-old rats(MSCs 3control);2)MSCs from 3-month-old ovariectomized rats(MSCs 3ovx);3)MSCs from 6-month-old rats(MSCs 6control);4)MSCs from 6-month-old ovariectomized rats(MSCs 6ovx);5)MSCs from 3-month-old rats with ADI(MSCs ADI 3control);6)MSCs from 3-month-old ovariectomized rats with ADI(MSCs ADI 3ovx);7)MSCs from 6-month-old rats with ADI(MSCs ADI 6control);8)MSCs from 6-month-old ovariectomied rats with ADI(MSCs ADI 6ovx).The results showed that the number of lipid droplets and expression level of LPL mRNA in MSCs ADI 3ovx and MSCs ADI 6ovx were higher than those in MSCs ADI 3control and MSCs ADI 6control,respectively;that the number of lipid droplets and expression level of LPL mRNA in MSCs ADI 6control were higher than those in MSCs ADI 3control respectively.The results of this study suggested that the adipogenic potential of MSCs from both aged and ovariectomied osteoporotic rats were augmented.更多还原