【摘要】 实验研究了DL-天冬氨酸和L-赖氨酸通过不同的比例合成的一系列聚[DL-天冬氨酸-CO-L-赖氨酸](PAL),经过1H-NMR、FT-IR、X-Ray方法进行表征后确认了结构并证明该类聚合物有较好的规律性。PAL开环后合成α,β-聚[(N-羟丙基/氨乙基)-DL-天冬酰胺-CO-L-赖氨酸](PHAAL),通过对PHAAL在磷酸缓冲液(pH=7.4,0.01M)和酶(木瓜蛋白酶,胰蛋白酶)溶液中降解实验的研究,结果显示该类聚合物具有良好的降解能力。利用MTT方法测PHAAL在Hela,ECV-304,Bcap37细胞中的细胞毒性,实验结果表明PHAAL的细胞毒性较低。利用含溴乙啶(0.25μg/ml)的琼脂糖凝胶(1.0%,w/v)电泳检测PHAAL的DNA结合能力,发现赖氨酸聚合比例高的PHAAL结合DNA的能力较强。综合各种实验结果分析,PHAAL是一类可作为非病毒性基因载体的聚氨基酸类高分子材料。更多还原

【Abstract】 A series of Poly[aspartic acid-co-L-lysine](PAL) are copolycondensed by DL-aspartice acid and L-lysine with different ratios.Their constructions are identified by the spectra of ~1H-NMR,FT-IR,X-Ray).These spectra are proved to have good regularity of these copolymers.α,β-Poly[(N-hydroxypropyl/aminoethyl)-DL-Aspartamide-co-L-lysine](PHAAL) is synthesized by ring-opening poly[aspartic acid-co-lysine](PAL).PHAAL has good degradability in the phosphoric acid buffer solution(0.01 M,pH=7.4) in the enzyme solution(Papain,Trypsine).PHAAL appeared tobe low cytotoxicity in Hela,ECV-304,Bcap37 cell lines,which was quantified by MTT assay.The combination ability of PHAAL with plasmid DNA was evaluated by agarose gel electrophoresis with agarose gel(1.0% w/v) containing ethidium bromide(0.25 μg/ml).The PHAAL with higher ratios of lysine in the copolymers have higher ability of condensing DNA.In summary,PHAAL,the polyaminoacid materials, could be one kind of macromolecule materials tobeused as the non-viral gene vehicle.更多还原