【摘要】 采用微管吮吸技术测定大鼠肝癌细胞的黏弹性;研究了秋水仙素、细胞松弛素D以及两者混合作用后对于肝癌细胞黏弹性的影响。结果表明:用CD处理癌细胞后发现癌细胞的弹性系数K1明显下降。与对照组相比:微丝骨架被CD抑制后,在加入Col后肝癌细胞的弹性系数K1显著降低;而微管骨架被col抑制后,在加入CD后肝癌细胞的弹性系数K1、K2和μ无明显变化。提示在微管骨架系统完整的情况下,微丝对肝癌细胞的黏弹性系数的的影响起主要作用。而微管骨架系统受到破坏后,微丝需借助于微管网络的作用来影响细胞的黏弹性。本研究对揭示癌细胞中骨架系统之间的交互作用对于细胞黏弹性的影响提高实验依据。更多还原

【Abstract】 In this study,we adopt micropipe technology to mensurate the viscoelasticity of hepatoma carcinoma cell from rat,and then research the influence of colchicincs(COL),cytochalasin D(CD),and the mixture of both drug on hepatoma carcino- ma cell of viscoelasticity.The result shows that:after use CD,the viscoelasticity coefficient K1 of cancer cell decrease apparently. After the microfilament backbone was CD inhibition,then after addition COL.,the hepatoma carcinoma cell of viscoelasticity co- efficient K1 decrease apparently.But after microtubule backbone was COL inhibition,then after addition CAD,the hepatoma carci- noma cell viscoelasticity coefficient K1、K2 and don’t marked chang.Under microtubule system is existing of Integrity.The interac- tion of microfilament on the hepatoma carcinoma cell of viscoelasticity coefficient.After the microtubule backbone system get breakdown.The interaction of the most important hypothesis:the microfilament must turn to microtubule contribution of lattice take the effect on viscoelasticity of cell.The interaction of This decision and reveal backbone system of cancer cell take the effect on viscoelasticity of cell.更多还原