【摘要】 目的:对实验性动脉粥样硬化家兔血清中巨噬细胞移动抑制因子、肿瘤坏死因子、白介素-6含量进行分析,以探讨它们在冠心病发病过程中的意义。方法:研究对象为新西兰大白兔,随机分成对照组(10只)和实验组(10只)。对照组喂食基础饲料,实验组喂食高脂饲料(基础饲料中加入1%胆固醇+5%猪油)。均喂饲8周后采用ELISA法检测其血清MIF、TNF-α、IL-6水平。结果:组织病理切片结果显示实验组兔主动脉弓内膜增厚,动脉粥样斑块明显。与对照组比较,实验组MIF、TNF-α、IL-6水平均升高,其差别有显著性(p＜0．01)。结论:MIF、TNF-α、IL-6与动脉粥样硬化的发生有密切关系,这些细胞因子可通过相互诱导、相互协同共同参与冠心病的发生、发展过程。更多还原

【Abstract】 Objective:To study the roles of MIF(macrophage migration inhibitory factor),TNF-αand IL-6 in atherosclerosis(AS).Methods:Twenty New Zealand rabbits were randomly divided into two groups.The rabbits in control group were given basic food and the rabbits in experimental group were given high lipid diet.After 8 weeks,the levels of serum MIF,TNF-αand IL-6 were determined by ELISA in the two groups.Results:Histological analyses showed that compared with control group,there were thicker intima in experimental group,in which,the rabbits treated with high lipid created mature athero- matous plaque,with higher levels of MIF,TNF-αand IL-6(p＜0.01).Conclusion:Rabbits can be used as an ideal model for re- searching atherosclerosis.There is close relationship between serum MIF,TNF-αand IL-6 levels and development of atherosclero- sis,which play an important role in the pathogenesis through mutual induction and synergistic actions.更多还原