【摘要】 目的:研究坐骨神经结扎损伤后疼痛受体P2X3在相应背根神经节(dorsal root ganglia,DRG)内的表达变化情况。方法:选取健康成年SD大鼠35只,建立右侧坐骨神经结扎损伤模型,采用免疫组织化学和图像分析技术检测相应L4-6DRG内P2X3的表达情况。结果:正常大鼠L4-6DRG内有大量P2X3免疫阳性神经元,坐骨神经结扎后3d P2X3表达即下调,3,7,14,21和28d其表达呈进行性下降趋势,各时间点与正常和假手术对照比较差异均有统计学意义(P＜0．05)。结论:坐骨神经结扎后P2X3在L4-6DRG内表达明显下调,提示其可能在神经源性疼痛中发挥一定的作用。更多还原

【Abstract】 Objective:To investigate the expression changes of P2X3 in dorsal root ganglia(DRG)after sciatic nerve ligation.Methods:35 healthy adult SD rats underwent right sciatic nerve ligation.Immunohistochemistry and image analysis were used to detect the expression of P2X3 in L4-6DRG.Results:There were abundant P2X3-positive primary sensory neurons in normal IA-6DRG.The expression of P2X3 was down-regulated 3 days after sciatic nerve ligation.It also showed that a decreasing tendency appeared during the period of 3-21 days.The expression did not return to the normal level after 28 days. Conclusion:The obvious down-regulation of P2X3 expression in L4-6DRG after sciatic nerve ligation indicates that it may play an important role in neuropathic pain sensation.更多还原