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一株中度嗜热嗜酸细菌的分离与分子鉴定
Isolation and Molecular Identification of A Strain of Moderately Thermophilic and Acidophilic Bacterium
【摘要】 用稀释分离法,从云南某温泉分离得到一株中度嗜热嗜酸细菌YN06。该菌株短杆状,革兰氏阴性,菌体大小为(0.3-0.5)μm x(1-2.2)μm。16S rDNA和16S—23S间隔区序列分析表明,YN06与嗜酸硫杆菌属的喜温硫杆菌处于同一进化树分支中,相似性均高达99%以上,因此该菌株被鉴定为喜温硫杆菌。
【Abstract】 By dilution method,a moderately thermophilic acidophilic bacterium YN06 was isolated from a hot spring of Yunnan province in China.The cells of the strain YN06 were Gram negative and rod-shaped in (0.3-0.5)μm x(1.0-2.2)μm.The 16S rDNA and 16S-23S intergenic spacer sequences analysis indicated that the strain YN06 was closdly relative to A.caldus with more than 99% sequence similarity.Thus the strain YN06 was identified as the species A.caldus.
【关键词】 中度嗜热嗜酸细菌;
16S rDNA;
16S—23S间隔区;
序列分析;
【Key words】 A moderately thermophilic acidophilic bacterium; 16S rDNA; 16S-23S intergenic spacer; Sequence analysis;
【Key words】 A moderately thermophilic acidophilic bacterium; 16S rDNA; 16S-23S intergenic spacer; Sequence analysis;
【基金】 国家自然科学基金创新群体(50621063);国家重大基础研究项目973(2004CB619201)
- 【文献出处】 现代生物医学进展 ,Progress in Modern Biomedicine , 编辑部邮箱 ,2007年05期
- 【分类号】Q93-33
- 【被引频次】2
- 【下载频次】224