【摘要】 从我国三大铜矿的酸性矿坑水中富集分离出9个具有较强活性的嗜酸氧化亚铁硫杆菌菌株,经过Cu²⁺的系列浓度梯度的培养,选出其中天然抗铜能力最强的菌株26^#,在Cu²⁺浓度为0．20mol/L的9K培养基中能在72h内完全氧化培养基中的Fe²⁺,在含0．22mol/L Cu2²⁺的9K培养基中能在192h内完全氧化培养基中的Fe²⁺。以CuSO₄·5H₂O为单变量驯化介质驯化该26^#抗铜菌株,26^#驯化菌株的Fe²⁺氧化能力明显增强:在含0．25mol/LCu²⁺的9K培养基中能在84h内完全氧化其中的Fe²⁺。为了提高驯化菌的稳定性,将驯化后的26^#菌株用紫外线进行诱变。研究结果表明:驯化诱变对菌种的改良有重要的作用,诱变后菌株的生长性能稳定,氧化活性进一步提高,26^#驯化诱变菌在0．25mol/LCu²⁺存在的条件下完全氧化9K培养基中Fe²⁺的时间约为60h,对Fe²⁺氧化能力明显强于驯化菌及野生菌。更多还原

【Abstract】 The 9 highly active Acidithiobacillus ferrooxidans（At.ferrooxidan）strains were enriched and isolated from acidic drainage water of three copper mines in China.Through a series of incubation using 9K medium containing varies of concentrations of copper ions,isolated 26# out of 9 strains was screened due to the highest resistance capacity to copper ions.The bacteria completely oxidized ferrous ions of 9K medium containing 0.20mo1/L of copper ions within 72h and completely oxidized ferrous ions of 9K medium containing 0.22mol/L of copper ions within 192h.The isolated 26# was adapted using 9K medium containing copper ions and the oxidation capacity of ferrous ions of adapted bacteria increased greatly:it completely oxidized ferrous ions of 9K medium containing 0.25mol/L of copper ions within 84h.Through ultraviolet irradiation induced mutation,the growth speed and oxidation capacity of isolate 26^# improved greatly under 9K medium containing 0.25mol/L of Cu²⁺ and the period of complete oxidizing ferrous ions was about 60h,which indicated that the mutant 26^# strain was more stable and its oxidation capacity of ferrous ions of was much higher than the adapted and the wild one.更多还原