【摘要】 目的:对纳米级Fe3O4磁性粒子与人肝癌细胞HepG-2及人正常肝细胞L02作用的生物学行为进行实验研究。方法:通过化学沉淀法制备粒径为10nm左右的纳米级Fe3O4磁性粒子,观察其表征;将不同浓度纳米级Fe3O4粒子加入培养液分别与HepG-2混合培养检测凋亡坏死率;将相同浓度粒子分别与HepG-2和L02混合培养,对两者作用的差异进行动态观察比较。结果:纳米级Fe3O4磁性粒子能在肝癌细胞HepG-2细胞内稳定存在72小时以上,有良好的生物相容性;透射电镜观察到Fe3O4磁性粒子主要分布于细胞的溶酶体及吞噬泡内。共培养1小时后即有较多的纳米磁性粒子进入HepG-2内,而3小时后才见L02细胞内有少量的磁性粒子进入。结论:此实验结果为磁性纳米粒子与肿瘤细胞微观结构的作用提供了有意义的实验数据,并可能对应用磁性纳米粒子治疗恶性肿瘤提供有价值的依据。更多还原

【Abstract】 Objective: To study magnetic nanoparticles biological behaviour on human liver cancer line HepG—2 and human normal liver cell line L02 in vitro. Methods: Magnetite nanoparticles whose diameter is about 10nm were prepared with chemical precipitation methods. Then their exosyndrome were observed. Both human liver cancer cell line HepG? and human normal liver cell line L02 were cultured respectively in culture medium contained Fe3O4 nanoparticles, then we observed transient changing and their differences. Result: The magnetite nanoparticles had favourable biocompatibility and were distributed in cytolysosome and phagocytotic vesicle. They could stay stably in HepG—2 for more than 72h. Under the same conditions, human liver cancer line HepG—2 take up a lot of nanoparticles within 1 hour, while human normal liver cancer cell line L02 take up only little nanoparticles after 3 hours. Conclusion: The results offer valuable data for the effect of magnetic nanoparticles on microstructure of tumor cell. They probably also offer valuable evidence for the application of magnetic nanoparticles on malignant tumor therapy.更多还原