【摘要】 目的:研究载脂蛋白E(ApoE)基因多态性与早发冠心病CHD)的关系。方法:应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)基因分析方法,测定92例早发CHD、237例迟发CHD患者和220名对照者的ApoE基因型;血脂水平按常规方法测定。结果:发现的5种ApoE基因型,分别为E3/3、E2/2、E3/2、E4/3及E4/2。早发CHD组和迟发CHD组ApoE 4/3基因型和ε4等位基因频率均高于对照组(P＜0．01);进一步对两组CHD患者的ApoE多态性进行分析,发现早发组ε4等位基因频率较迟发组高(P＜0．05)。ApoE各等位基因型之间,TC和LDL-C水平之间存在统计学差异(P＜0．05)。结论:ApoE基因多态性与早发CHD的发生发展有关。更多还原

【Abstract】 Objective:To detect the association of apolipoprotein E (apoE) gene polymorphism with early-onset coronary heart disease (GHD) and to evaluate the effect of apo E gene polymorphism.Methods:The apo E genotypes of 92 early-onset GHD patients (ranged from 38 to 55 years),237 delayed CHD patients and 220 controls were determined by PCR-RFLP.Plas- ma lipid levels were measured by using routine methods.Results:Five genotypes of apo E,E3/3,E2/2,E3/2,E4/3 and E4/2 were detected in this study.There were Statistical differences in apoE4/3 andε4 frequencies between early-onset and delayed patients and controls (P＜0.05).The frequency ofε4 in the early-onset CHD patients was higher than that in the delayed GI-ID patients.ε4 andε2 alleles also differently affected the plasma total cholesterol and low density lipoprotein cholesterol levels.Conclusion:These results suggest that apo E gene polymorphisms is much concerned with the occurrence and development of early-onset CHD.更多还原