【摘要】 比较了高氯化铁,硫酸亚铁,甘氨酸,L-谷氨酸钠以及脲(尿素)5种化学试剂对商品果胶酯酶中果胶酯酶(PE)和聚半乳糖醛酸酶(PG)的酶活抑制效果,发现高氯化铁,硫酸亚铁,和L-谷氨酸钠对聚半乳糖醛酸酶酶活均具有明显且相似的抑制作用,在一定的浓度范围内,随着浓度的增加,其抑制效果逐渐增强,超过此浓度,其抑制能力不再增强。而甘氨酸并不具有很好的抑制能力,对于果胶酯酶来说,上述几种试剂对其都没有抑制作用。脲(尿素)对两种酶均有抑制作用,其对聚半乳糖醛酸酶酶活的抑制作用要强于果胶酯酶。更多还原

【Abstract】 The inhibiting effect on Pectinesterase (PE) and Polygalacturonase (PG) activity of commol/Lercial pectinase preparation by Iron(Ⅲ) chloride hexahydrate, Iron(Ⅱ) sulfate heptahydrate, glycine, L-Glutamic acid sodium salt and urea was studied. Iron(Ⅲ) chloride hexahydrate, Iron(Ⅱ) sulfate heptahydrate and L-Glutamic acid sodium salt have the obvious and similar inhibiting effect: their inhibiting abilities increased companied with their concentration in a certain range and kept unalterable out of the range. Glycine has unstable inhibiting abili-ty. Above four reagents have no inhibiting effect on PE. Urea has the inhibiting effect on two enzymes and the effect on PG is more stonger.更多还原