【摘要】 采用高效液相色谱法,选择大连依利特氨基色谱柱,以乙腈∶水=80∶20为流动相,示差折光仪为检测器,外标法进行定量分析,测定塔格糖含量,所得结果良好,相对标准偏差为0.10%(n=5),线性相关系数为0.9995。塔格糖的最小检测量在微克级。更多还原

【Abstract】 Tagatose was quantitatively determined by high performance liquid chromatography with Dalian Elite column, a mixture of CH3CN-H2O(80∶20, volume ratio) as mobile phase and refractive index. There was a good linear relationship with in the range of 5~40μg (r=0.9995) and relative standard deviation was 0.12%(n=5). The detection limit was 25μg. The method is accurate and is proposed to be used for quality control of tagatose.更多还原