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我国引进萨罗罗非鱼群体的AFLP遗传指纹
AFLP fingerprinting of the introduced stock of Sarotherodon melanotheron in China
【摘要】 应用AELP技术初步分析了我国引进的萨罗罗非鱼群体的遗传指纹特征,8对选择性引物在25个个体中共检测到247个扩增片段,每对引物扩增出的片段数目为27-34,平均扩增片段数目为30.9。群体的平均多态位点比例为16.6%。共检测出25个基因型,每个个体含有独自的基因型。群体内个体间的遗传相似度为0.929-0.995,群体遗传变异较小。本研究结果可为我国萨罗罗非鱼的种质保存与管理提供科学依据。
【Abstract】 The genetic fingerprinting of the introduced stock of Sarotherodon melanotheron in China was analyzed using AFLP technique.A total of 247 fragments were detected in the 25 samples using eight primer pairs,the number of fragments produced by each pair of primers were 27-34,the average fragments was 30.9,the average polymorphic loci of this stock were 16.6%.25 genotypes were found with each sample owning one genotype.The genetic similarities of the introduced stock of S.melanotheron varied form 0.925 to 0.995.These results could be served as genetic base for its management and conversation.
【Key words】 Sarotherodon melanotheron; AFLP; genetic fingerprinting; genetic variation;
- 【文献出处】 上海水产大学学报 ,Journal of Shanghai Fisheries University , 编辑部邮箱 ,2007年03期
- 【分类号】S917.4
- 【被引频次】9
- 【下载频次】173