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我国引进萨罗罗非鱼群体的AFLP遗传指纹

AFLP fingerprinting of the introduced stock of Sarotherodon melanotheron in China

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【作者】 赵金良王伟伟李思发李学军赵丽丽

【Author】 ZHAO Jin-liang1,2,WANG Wei-wei1,LI Si-fa1,LI Xue-jun1,ZHAO Li-li1(1.Key Laboratory of Aquatic Genetic Resources and Aquacultural Ecosystem Certificated by the Ministry of Agriculture,Shanghai Fisheries University,Shanghai 200090,China;2.Key Laboratory of Aquatic Heredity Breeding & Breed Biology,Ministry of Agriculture,Freshwater Fisheries Research Center,Chinese Academy of Fisheries Sciences,Wuxi 214081,China)

【机构】 上海水产大学农业部水产种质资源与养殖生态重点开放实验室上海水产大学农业部水产种质资源与养殖生态重点开放实验室 上海200090中国水产科学研究院淡水渔业研究中心农业部水生动物遗传育种与养殖生物学重点开放实验室江苏无锡214081上海200090

【摘要】 应用AELP技术初步分析了我国引进的萨罗罗非鱼群体的遗传指纹特征,8对选择性引物在25个个体中共检测到247个扩增片段,每对引物扩增出的片段数目为27-34,平均扩增片段数目为30.9。群体的平均多态位点比例为16.6%。共检测出25个基因型,每个个体含有独自的基因型。群体内个体间的遗传相似度为0.929-0.995,群体遗传变异较小。本研究结果可为我国萨罗罗非鱼的种质保存与管理提供科学依据。

【Abstract】 The genetic fingerprinting of the introduced stock of Sarotherodon melanotheron in China was analyzed using AFLP technique.A total of 247 fragments were detected in the 25 samples using eight primer pairs,the number of fragments produced by each pair of primers were 27-34,the average fragments was 30.9,the average polymorphic loci of this stock were 16.6%.25 genotypes were found with each sample owning one genotype.The genetic similarities of the introduced stock of S.melanotheron varied form 0.925 to 0.995.These results could be served as genetic base for its management and conversation.

【基金】 农业部水生动物遗传育种与养殖生物学重点开放实验室开放课题(2003-2005);上海市重点学科建设项目(Y1101)
  • 【文献出处】 上海水产大学学报 ,Journal of Shanghai Fisheries University , 编辑部邮箱 ,2007年03期
  • 【分类号】S917.4
  • 【被引频次】9
  • 【下载频次】173
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