【摘要】 研究了三氯氧磷辅助下L-羟脯氨酸的成肽反应,建立了采用反相高效液相色谱-质谱/质谱联用技术分离鉴定羟脯氨酸寡肽混合物的方法,优化了L-羟脯氨酸寡肽混合物的色谱分离条件。实验以YWG C8柱(10μm,250mm×10mm)为分离柱,以乙腈-0.06%三氟乙酸水溶液(体积比为2∶98)为流动相进行等度洗脱,在正离子模式下对洗脱物进行了电喷雾电离串联质谱鉴定。结果显示,分离出的各组分分别为L-羟脯氨酸二肽、L-羟脯氨酸环二肽和L-羟脯氨酸三肽。更多还原

【Abstract】 A reversed-phase high performance liquid chromatographic-electrospray ionization tandem mass spectrometric method(RP-HPLC/MS2) was developed for the analysis and identification of a reaction mixture of L-hydroxyproline oligo-peptides.The mixture was separated on a YWG C8 column(10 μm,250 mm×10 mm) using acetonitrile-water containing 0.06% trifluoroacetic acid(2∶98,v/v) as the mobile phase.The separation conditions were optimized.The mixture was analysed with electrospray ionization mass spectrometry(ESI-MS) and ESI-MS/MS in positive mode.MS and MS2 spectra of individual chromatographic peak were obtained.It indicated that there were hydroxyproline dipeptide,hydroxyproline tripeptide and hydroxyproline cyclo-dipeptide in the mixtures,besides hydroxyproline.The described method can be effectively applied to perform identification of hydroxyproline oligo-peptide mixtures.更多还原