【摘要】 对根霉经发酵产凝乳酶的发酵条件进行研究。结果表明,发酵培养基的最佳组成为4.5%的米粉麸皮(按2:3的比例)水解液、4.0%的黄豆粉水解液、0.3%的CaCl2、0.5%的KCl。摇瓶培养中培养基起始pH值为4.5,培养时间、温度分别为60h、34℃时,所产凝乳酶活力达161.14SU/mL且凝乳酶活力与蛋白酶活力的比值最大为27.88。更多还原

【Abstract】 The fermentation conditions for producing chymosin with rhizopus have been investigated in this paper. The results indicated that the optimum fermentation medium composition is: 4.5 % rice noodle bran (according to 2:3 proportion) hydrolysates, 4.0 % soy flour hydrolysis fluid, CaCl2 0.3 % and KCl 0.5 %. In the shake culture pH value is 4.5 at the beginning; the chymosin activity will be as high as 161.14 SU/mL when the reaction time and temperature respectively are raised to 60 h and 34 ℃. The activity ratio of chymosin and proteinase arrives 27.88 at the same time.更多还原