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甘薯叶可溶性蛋白提取工艺研究
STUDY ON THE BROWNING OF DANGSHAN PEAR JUICE CONCENTRATES DURING PROCESS
【摘要】 以甘薯鲜叶为原料,研究了碱提酸沉法提取甘薯叶可溶性蛋白的工艺,通过单因素和正交实验,确定出提取甘薯叶可溶性蛋白的最佳工艺条件:提取液(0.05%NaHSO3)pH8.0,料液比(w/v)1∶4,打浆时间3min,酸沉pH4.5。该优化条件下可以得到纯度为50%~65%的甘薯叶可溶性蛋白,可溶性蛋白提取率为14%~18%。
【Abstract】 This work was focus on the study of browning of Dangshan pear juice concentrates during process. Colour development through the whole proess was measured by color index T440 ,total phenols,total amino acid,polyphenoloxidase, 5-hydroxymethyl furfural (5-HMF) and other facts .The results showed that during the process, the browning was mainly enzymtic before pre- concentrates; non-enzymatic and enzymatic browning contributed together between pre- concentrate and ultra filter; after ultra filter it was non-enzymatic completely.
【关键词】 甘薯叶;
可溶性蛋白;
提取工艺;
【Key words】 Dangshan pear juice concentrates; process; enzymatic browning; non-enzymatic browning;
【Key words】 Dangshan pear juice concentrates; process; enzymatic browning; non-enzymatic browning;
【基金】 科技部科研院所社会公益研究专项(2002005DIA4J035-3)
- 【文献出处】 食品研究与开发 ,Food Research and Development , 编辑部邮箱 ,2007年03期
- 【分类号】TQ936
- 【被引频次】20
- 【下载频次】712