【摘要】 利用现代技术微波、超声波及传统技术萃取古尼虫草总黄酮,采用正交试验设计筛选出提取古尼虫草总黄酮的最佳方法与条件。铝盐比色法测定总黄酮含量结果表明三种提取方法的最佳条件分别为:(1)微波萃取:90%乙醇,料液比1:25,功率160W,提取1.5min;(2)超声波萃取:85%乙醇,料液比1:30,80℃水浴,10min,提取1次;(3)传统萃取:85%乙醇,料液比1:20,65℃水浴中,提取2次,每次120min。不同方法总黄酮的最高提取率具有显著性差异(P<0.05)分别是:微波萃取率2.99%;超声波萃取率:6.56%;传统萃取率3.94%。结果证明:超声波萃取法具有高效、快速的特点,其提取率与银杏根中总黄酮含量相近,说明古尼虫草也是黄酮的重要来源之一。更多还原

【Abstract】 Cordyceps gunnii is one of the most potential epiphyte-resources in medicine due to various bioactive compounds. In this paper, extraction of total flavonoids in Cordyceps gunnii was investigated by microwave , ultrasonic and tradition methods. The most optimum method and condition was selected using orthogonal design. An amount of total flavonoids was determined using Aluminium salt UV-Vis spectrophotometry. The most optimum condition of extraction from the results of orthogonal design for an amount of total flavonoids in Cordyceps gunnii included: (1) extraction of microwave was ratio of material and 90 % ethanol liquor(1:25), power (160 W) and time (1.5 min);(2)extraction of ultrasonic was 85 % ethanol,ratio of material and ethanol liquor (1:30), temperature(80 °C)in water bath for 10 min and repeated once; (3) extraction of tradition was 85 % ethanol, ratio of material and ethanol liquor (1:20), temperature (65 °C) in water bath , time (120 min) and repeated two times. The highest ratio of total flavonoids extraction using three methods was significantly difference (P<0.05), which was 2.99 % (microwave method), 6.56 % (ultrasonic method) and 3.94 %(traditional method), respectively.更多还原