【摘要】 本实验主要研究了从白葡萄酒废酵母中提取蛋白和多糖的方法。结果表明:经处理后的酵母悬浮液在超声波功率600W,作用时间4s,间歇时间2s,作用90次的条件下,促溶效果最好。经超声波处理后,再添加0.6%(W/V)的木瓜蛋白酶,在55℃,pH5.5下,反应时间为30h,提取液中蛋白含量达到9.84g/L。提取蛋白后的残渣,用碱法提取多糖,含量达58.69%。更多还原

【Abstract】 The extracted techniques of protein and polysaccharide from white wine waste yeast were studied.The results showed:The best disaggregated effect of yeast suspension by ultrasonic is disposed in 600W,acting 4s,interval 2s,90 times.Then,put 0.6% papain into the yeast suspension which treat with ultrasonic,pH 5.5,55 ℃,reaction 28 h,the protein content of the extraction was 9.84 g/L.The polysaccharide obtained from the residue which extracted protein by NaOH solution.The polysaccharide content of extraction was 58.69%.更多还原