【摘要】 以蛋壳膜为原料,采用稀碱与酶解相结合的方法提取硫酸软骨素。以胰蛋白酶、胃蛋白酶分解去除杂蛋白,经酒精沉淀并干燥后得到产品。实验得到的最佳提取工艺为:料液比1:1.5,碱浓度5%,碱提温度40℃,胰蛋白酶、胃蛋白酶的用量分别为1.5%、0.8%,pH值分别为8.2和5.5,酶的各自最适条件作用时间均为2.0h。更多还原

【Abstract】 Chondroitin sulfate was extracted from the eggshell membrane with a method of dilute alkali-enzyme hydrolysis.The product of chondroitin sulfate was gotten after ethanol disposition and desiccation by eliminating the protein with trypsin and pepsin.The results showed the optimum conditions of weight proportion 1:1.5(material:liquid),alkali concentration 5%,temperature 40 ℃.Dosage of trypsin and pepsin 1.5% and 0.8% respectively,pH value 8.2 and 5.5 respectively and reaction time 2.0 h respectively.更多还原