【摘要】 本实验研究了热水浸提法提取杏鲍菇粗多糖的最佳工艺流程及工艺条件。用热水浸提杏鲍菇多糖,乙醇沉淀和Sevage法脱蛋白得到初步纯化的多糖,采用硫酸-苯酚法测定多糖的含量。在单因素试验的基础上,通过正交试验进一步优化提取工艺条件,确定影响杏鲍菇多糖得率的主次因素分别为提取温度、提取时间、料液比和乙醇浓度。结果表明,提取最优工艺为:时间3h,水温为90℃,乙醇的体积为提取液体积的70%,料液比为1:20时多糖沉淀量最大。此工艺条件下杏鲍菇多糖的得率为2.88%。更多还原

【Abstract】 The optimal craft process of extracting polysaccharides from pleurotus eryngii by hot water was studied in this paper.The preliminarily purified polysaccharides were obtained with the step of being leached by hot water,precipitated by ethanol and eliminated protein with Sevag method.The content of polysaccharides was determined by colourimetry using sulfuric acid and phenylic acid.Then the optimal conditions were obtained by orthogonal test on the basis of single factor test.The results indicate that the optimal conditions by orthogonal test are as following:time 3 hour,the temperature of water 90℃,the volume of ethanol is 70% compared to the volume of extracting solvent and the ratio of water to material is 1:20.At this time,the maximal yield of polysaccharides is 2.88%.更多还原