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超声波提取翻白草中熊果酸工艺优化研究

Study on Optimization of Extraction Process of Ursolic Acid from Potentilla discolor Bge with Ultrasonic

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【作者】 李胜华伍贤进郁建平王丽娜胡美忠

【Author】 LI Sheng-hua1,WU Xian-jin1,*,YU Jian-ping2,WANG Li-na1,HU Mei-zhong2 (1.Department of Bioengineering, Huaihua College, Huaihua 418008, China; 2.Institute of Biochemistry and Nutrient, Guizhou University, Guizhou 550025, China)

【机构】 怀化学院生物工程系贵州大学生化营养研究所贵州大学生化营养研究所 湖南怀化418008湖南怀化418008贵州贵阳550025

【摘要】 目的:筛选翻白草中熊果酸的最佳提取工艺。方法:采用超声波提取、正交试验优化及高效液相色谱法测定所得翻白草中熊果酸的含量。结果:翻白草中熊果酸的提取最佳工艺是乙醇浓度为75%,固液比为1:60,提取时间为60min,超声波功率为610W,各因素的影响依次为提取时间>固液比>乙醇浓度和超声波功率。结论:最佳优选结果用于验证实验具有较好的重现性。

【Abstract】 Objective: To screen optimum the extraction process of ursolic acid from Potentilla discolor Bge. Methods: ultrasonics extraction was used to extract. The optimum extraction process was confimed by the orthogonal test. The content of ursolic acid in Potentilla discolor Bge. was determined by HPLC. Results: The optimum conditions of ultrasonics extraction are as follow: ethanol concentration 75%, time 60 min, ratio of material to solution 1:60, and power of extraction 610 W. The effects of factors on yield decrease progressively in the following order: extracting time>ratio of material to solution>ethanol concentration>power of extraction. Conclusion: The best optimized process has a good repeatability confimatory experiment.

【关键词】 翻白草熊果酸提取
【Key words】 Potentilla discolor Bge.ursolic acidextraction
【基金】 科技部国家科技基础条件平台项目(2004DKA30430);湖南省普通高校学科带头人培养项目
  • 【分类号】R284
  • 【被引频次】24
  • 【下载频次】529
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