【摘要】 目的:研究和建立食品中绿脓杆菌的检测方法。方法:通过比较不同增菌液增殖效果,分离培养基的分离效果和确定生化项目建立检验方法,并通过制备人工污染样品验证方法的可行性。结果:确定了SCDLP为增菌液,C-F-C和C-N为分离培养基,以O/F葡萄糖、氧化酶为主的生化鉴定项目,以及MPN法作为定量方法。结论:本方法适用于食品中绿脓杆菌定性和定量检测。更多还原

【Abstract】 Objetive: Studied and established the method for detection of Pseudomonas aeruginosa in food.Method: By making comparison of the effects of different enrichment broth and isolation media, and confirming the tests of physiology and biochemistry to establish the method. Then we validated the feasibility by detecting the artificially contaminated food.Result: Comfired SCDLP as the enrichment broth, C-F-C and C-N as the isolation media, some tests like O/F glucose, oxidase for physiology and biochemistry identification, MPN as the method of quantitative detection.Conclusion: This method is suitable for thequalititave and quantitative detection of Pseudomonas aeruginosa in food.更多还原