【摘要】 本实验选用截留分子量为100kD的中孔纤维超滤膜从免疫牛初乳乳清中分离提取IgG,研究了操作压力和温度在超滤过程中对膜透过速率的影响,确立了最适操作压力为0.15MPa,最适操作温度为25℃;并采用分段超滤法对免疫牛初乳乳清中的IgG进行分离,从而使浓缩液中IgG纯度得到进一步提高。更多还原

【Abstract】 IgG was separated and extracted from immune bovine colostrums whey by hollow-fiber membrane ultrafiltration (100 kD, molecular, cut-off). The factors affecting separation such as pressure and temperature were studied. The results showed that optimum operating pressure and temperature were 0.15 MPa and 25 ℃ respectively, and the purity of IgG in concentrated liquid could be further improved by subsection ultrafiltration.更多还原