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碱溶性银耳粗多糖的提取及其清除自由基作用的研究

Study on Extraction and Antioxidant Effects of Crude Alkai-soluble Tremella Polysaccharide

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【作者】 吴琼郑成宁正祥刘本国董华强

【Author】 WU Qiong1,ZHENG Cheng2, NING Zheng-xiang1,LIU Ben-guo1,DONG Hua-qiang3 (1.College of Light Industry and Food Technology, South China University of Technology, Guangzhou 510640, China; 2.School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou 510091, China; 3.Department of Food Science, Foshan University, Foshan 528231, China)

【机构】 华南理工大学轻工与食品学院广州大学化学与化工学院佛山科学技术学院食品系 广东广州510640广东广州510091广东广州510640广东佛山528231

【摘要】 本实验研究了碱溶性银耳粗多糖(crude alkai-soluble tremella polysaccharide,CATP)的提取及其清除自由基的作用。利用响应面软件进行实验设计,确定最佳提取方案为:提取时间3.56h,NaOH浓度0.76mol/L,液固比81.89。同时考察了碱溶性银耳粗多糖和水溶性银耳粗多糖(tremella polysaccharide,TP)对DPPH(二苯代苦肼基自由基)、羟基自由基、超氧自由基的清除作用。结果表明,碱溶性银耳粗多糖具有良好的抗氧化能力,并且随着多糖浓度的增高抗氧化性增强。

【Abstract】 It’s mainly studied on extraction and antioxidant effects on the role of crude alkai-soluble tremella polysaccharide(CATP). The optimum extracting conditions of CATP were as follows: extraction time 3.56 h, NaOH concentration 0.76 mol/L, solvent/solid ratio 81.89. The antioxidant effects of CATP and TP(tremella polysaccharide) were studied by scavenging DPPH, hydroxyl and superoxide anion free radical. The results showed that CATP has better antioxidant capabilities than TP.

【关键词】 银耳碱溶性粗多糖提取自由基
【Key words】 Tremella fuciformis BerkCATPextractionfree radical
  • 【分类号】R284;R285
  • 【被引频次】73
  • 【下载频次】980
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