【摘要】 本实验主要研究湖桑(Morus alba L.Husang)的粗老桑叶中酸性蛋白多糖的提取、分离、纯化及结构鉴定。水煎法提取桑叶粗多糖,D-101大孔树脂柱层析,脱蛋白,脱色,再上DEAE纤维素(DE-32)柱及Sephadex G-100葡聚糖凝胶柱对产物进行分离纯化,得HPLC单一峰的桑叶多糖纯品。通过单糖组成分析、酸性基团含量、IR、UV、HPLC及氨基酸组分测定等方法解析其结构及理化性质。实验结果显示:该多糖分子量达50万,多糖主链主要为α-型吡喃糖连接,由D-鼠李糖、L-阿拉伯糖、D-果糖、D-葡萄糖、D-半乳糖组成,摩尔比为8.91:2.71:1.00:3.75:6.04;该多糖糖醛酸含量为5.33%,硫酸根含量为8.03%;其蛋白质部分占多糖总量的0.83%;含17种氨基酸,其中7种为人体必需氨基酸。表明该多糖为酸性蛋白多糖(acid proteoglycan from Folium mori,APFM)。更多还原

【Abstract】 This experiment studied the extraction, separation, purification and physico-chemical characteristics of acid proteoglycan extracted from Folium mori(APFM). The polysaccharides were extracted from F.mori by water extraction, and then isolated and purified by D101resin, DEAE-cellulose column and SephadexG-100 gel column chromatography successively. Monosaccharides composition analyses, acidic groups contents, and amino acids compositions were assayed with IR, UV, HPLC to for deduce the structural and physical and chemical properties. Results showed that APFM is a pure acidic proteoglycan withits molecular weight over 500 Da. It was constituted by D-rhamnose, L-arabinose, D-fructose, D-glucose and D-galactose with molar ratio: 8.91: 2.71: 1.00: 3.75: 6.04 respectively; and the contents of uronic acid, sulfate radical and protein 5.33%, 0.83% and 8.03% respectively. Its monomer of backbone is α-pyranose. In addition, APFM contains seventeen kinds of amino acids and seven kinds of which are human essential .更多还原