【摘要】 研究了菌体培养时间、溶菌酶作用条件(酶作用温度、浓度和时间)对纳豆菌BNK菌株原生质体制备和再生的影响。在此基础之上,考察了不同再生培养基、渗透压稳定剂及原生质体保护剂对再生率的影响。确定了菌株BNK原生质体制备和再生最佳条件为:菌体培养9h收集,0．8mg/ml溶菌酶,30℃作用40min制备原生质体,将原生质体液涂布于以甘露醇为渗透压稳定剂、添加有淀粉的PYG培养基中再生,此时原生质体制备率达98．2%,再生率为28．4%。更多还原

【Abstract】 The effects of natto strain BNK cultivation time,operational times,temperature and concentration of lysozyme on protoplast production and regeneration were studied.In addition,different operational methods, regeneration media,osmotic stabilizers and protective additives were also investigated.The appropriate conditions of cultur are 9h,BNK treated by 0.8 mg/ml lysozyme for 40 min at 30℃,then spreaded on PYG medium with mannitol as stabilizer and starch as a protector.Under these conditions the protoplast formation achieves 98.2% and regeneration frequency 28.4%.更多还原