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利用电子自旋共振(ESR)研究啤酒酿造过程中的自由基

Study on Free Radicals Emerging during Beer Brewing by ESR Method

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【作者】 严敏李崎董建军单连菊顾国贤

【Author】 YAN Min1,LI Qi1,*,DONG Jian-jun2,SHAN Lian-ju2,GU Guo-xian1 (1.Key Laboratory of Industrial Biotechnology, Ministry of Education ,Southern Yangtze University , Wuxi 214036, China; 2.Research and Development Center, Tsingtao Brewery Co. Ltd., Qingdao 266061, China)

【机构】 江南大学教育部工业生物技术重点实验室青岛啤酒股份有限公司科研开发中心江南大学教育部工业生物技术重点实验室 江苏无锡214036江苏无锡214036山东青岛266061

【摘要】 本文利用电子自旋技术(ESR)考察了啤酒酿造各阶段,包括糖化、煮沸、发酵、过滤中自由基的形成与变化情况。发现煮沸阶段产生自由基最多,发酵阶段自由基含量有所下降,但过滤后又上升。发酵前后啤酒(麦汁)的曲线形态截然不同,说明麦汁所含抗氧化剂(自由基清除剂)种类与发酵后的啤酒不同,前者作用持久,主要在培育后期抑制自由基生成,后者作用迅速,主要在培育初期发挥清除自由基作用。糖化阶段发现的未报道过的新型自由基(暂名FR1)与·OH的形成和数量有密切关系。

【Abstract】 Brewing process including mashing, boiling, fermentation and filtration was studied by electron spin resonance(ESR) method in this paper. It was found that free radicals were yielded mostly during boiling phase, while a decline after fermentation and an increase after filtration occurred. Free radical generation courses of each phase before or after fermentation were different, especially between wort and beer, which meant different kinds of oxidants in them. The former has a persistent effect mainly inhibiting the antioxidant formation in late incubation, while the latter has a rapid effect of scavenging the antioxidant in the early incubation. A new-type free radical (FR1) is found in the saccharifying phase related intimately to the formation and quantity of ·OH.

【关键词】 自由基ESR啤酒酿造糖化
【Key words】 free radicalESR(electron spin resonance)beerbrewingmashing
  • 【分类号】TS262.5
  • 【被引频次】8
  • 【下载频次】365
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