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富硒绿茶功能成分的超声波提取技术及其抗氧化活性研究
Study on Ultrasonic-assisted Extraction and Antioxidant Activity of Se-enriched Green Tea Functional Components
【摘要】 采用超声波技术提取富硒绿茶功能成分,通过L9(34)正交试验对提取工艺进行优化,采用DPPH法和亚油酸体系法对富硒绿茶功能成分抗氧化活性进行了评价,并分析了化学组成。结果表明按照工艺:50%乙醇为溶剂,超声波功率250W,料液比为1:15,提取温度50℃,提取时间1.5h,重复提取两次,制得的富硒绿茶功能成分得率为43.76%,其抗氧化活性高于其他处理制得的提取物和阳性对照α-生育酚,其化学组成为:硒4.35±0.11μg/g,茶多酚(55.84±2.31)%,多糖(13.37±3.02)%,蛋白(25.75±4.05)%,咖啡碱(5.99±0.01)%。
【Abstract】 The ultrasonic was used to extract Se-enriched green tea functional components. The extraction pararmeters were optimized by L9(34) orthogonal design. And the composition of Se-enriched tea functional components was researched. The antioxidant activity of Se-enriched green tea functional components was assessed by FTC method and DPPH method. The optimized parameters were as follow: 50% (W/V) ethanol as solvent, ultrasonic power of 250W, ratio of solid/liquid of 1:15, extracting for 2 times at 50℃ for 1.5 hours each time. The yield was 43.76%. The antioxidant activity of the extracts under the optimized condition, which contained Selenium 4.35±0.11μg/g, tea polyphenol (55.84±2.31)%, polysaccharide (13.37± 3.02)%, protein (25.75±4.05)%, theine (5.99±0.01)%, was stronger than the extracts under other technology parameters and positivec ontrolα -tocopherol.
【Key words】 Se-enriched; ultrasonic; functional components; antioxidant;
- 【文献出处】 食品科学 ,Food Science , 编辑部邮箱 ,2007年01期
- 【分类号】TS272
- 【被引频次】47
- 【下载频次】907