米糠中阿魏酸的提取及高效液相色谱法测定

【摘要】 以提取液中阿魏酸的含量为指标,考察了碱溶液提取法、乙醇提取法、超声提取法、甲醇-甲酸提取法、碱醇提取法的优劣,用正交实验法对碱浓度、温度、时间、碱醇比和固液比5个因素进行研究,得到最佳工艺条件:1%氢氧化钠溶液与乙醇按4∶1的比例混和,80℃下提取6h,固液比为1∶8,加入0.2g/L的亚硫酸钠作为抗氧化剂。采用高效液相色谱法测定,色谱柱为C18ODS(4.6mm×150mm),流动相为甲醇-水-冰乙酸(35∶65∶0.9,v/v),流速1.0mL/min,柱温25℃,检测波长为322nm。上述条件下,阿魏酸在1.0～5.0μg/mL范围内线性关系良好,r=0.99928。该方法相对标准偏差在0.98%以内,平均回收率为99.7%。更多还原

【Abstract】 Total content of ferulic acid was taken as index to compare five extraction methods,including hydrolysis,reflux,ultrasonic.Base on the orthogonal design,five factors were optimized:concentration of sodium hydroxide,temperature,extraction time,sodium hydroxide/ethanol ratio and solid/liquid ratio.The optimized conditions are as follows:Sodium hydroxide,1%;temperature,80℃;extraction time,6h;Sodium hydroxide/ethanol ratio,4∶1;solid/liquid ratio,1∶8,adding 0.2g/L sodium sulfite as antioxidant.The chromatographic conditions included ODS-C18 column(4.6mm×150mm) and the mobile phase consisting of a mixture of methanol-water-acetic acid(35∶65∶0.9,volume ratio) at a flow rate of 1.0mL/min at 25℃.The detection wavelength were 322nm.There is a good linear relationship in the range of 1.0～5.0μg/mL for ferulic acid,and the correlative coefficient is 0.99928.The relative standard deviation is 0.98% and the average recovery is 99.7%.更多还原