【摘要】 不同杂交后代苹果54号、45号及富士苹果多酚氧化酶特性比较结果表明:不同苹果的最适pH值不同,45号和富士为5.5,54号为6.0;45号、54号和富士最适温度分别为30,35,40℃;富士热稳定性最强,其次是45号,最次为54号;不同苹果PPO的最适底物依次为绿原酸、咖啡酸、儿茶酚;Na2S2O3对PPO活性抑制效果最佳,L-半胱氨酸与抗坏血酸的抑制效果较好,EDTA-2Na的抑制效果较差;不同苹果PPO同工酶酶带不同,染色深浅不同。富士有四条酶带,45号和54号只有一条共有酶带,富士酶带数最多,染色最深。更多还原

【Abstract】 The PPO characteristics of 2 new cultivars （45 and 54 ）of different apple hydrids and Fuji apple were studied. The results indicated that the optimal pH of 45, 45 and Fuji are 6.0 and 5.5,respectively; the optimal temperature of 45、54 and Fuji are 30,35,40 ℃,respectively; the most suitable substrate is Chlorogenic acid; Na₂S₂O₃,V_C,L-Cysteine and EDTA -2Na could inhibit PPO activity, Na₂S₂O₃ is the most effective inhibitor for PPO of the apple; Isoenzyme patern of PPO is different, Fuji has 4 paterns, 45 and 54 only have 1patern.更多还原