【摘要】 将Streptoverticillium mobaraense发酵得到的谷氨酰胺转胺酶粗酶液进行凝胶层析,采用SDS-PAGE电泳得到了谷氨酰胺转胺酶的分子质量在37ku左右,pI值在8.7～9.0。对超滤过程TGase的酶活损失和超滤膜通量综合考虑,确定超滤过程温度控制在8℃左右比较适宜,在此基础上,考察了对膜通量有较大影响的酶液pH值和超滤压力等因素。实验结果表明,将料液pH调节到7.5左右,压力控制在0.20～0.25MPa比较理想。更多还原

【Abstract】 The crude transglutaminase broth fermented by Streptoverticillium mobaraense was separated and purified by gel chromatography.The SDS-PAGE results showed that the molecular weight of transglu- taminase and the value of pI were 37ku and 8.7～9.0 respectively.The main parameters of the ultra-filtration including temperature,pH value and pressure on the loss of the transglutaminase activity and membrane per- meating flux were analyzed and the results are(8±2)℃,7.5 and 0.20～0.25MPa respectively.更多还原