【摘要】 运用Box-Behnken中心组合响应面分析法优化纤维素酶法提取黄芪多糖的工艺条件,探讨了酶解过程中酶解pH值、温度和加酶量对多糖提取含量的影响。优化工艺方案为:酶解pH4.0,温度56.5℃,加酶量61U/ g,其多糖的平均含量达到20.31%。更多还原

【Abstract】 Box-Behnken center-united experiment design attached to response surface method was applied to optimize the extraction condition for Astragalus polysaccharides(APS)by cellulase.How the parameters which influence the content of APS,such as pH value,reaction temperature and enzymatic quantity were dis- cussed.The results indicated that the optimal parameters of extraction technology for the content of APS by cellulase were:pH value 4.0,reaction temperature 56.5℃,enzymatic quantity 61U/g.The maximal content of APS in average was 20.31% in this paper.更多还原